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Diagnogreen is 2-[7-1,1-Dimethyl-3-(4-sulfobutyl) benz[e] indolin-2-ylidene]-1, 3, 5-heptatrienyl]-1, 1- (dimethyl-3-(4-sulfobutyl)-1H-benz[e]indolium hydroxide, inner salt, sodium salt. Its empirical formula is C43H47N2NaO6S2 and molecular weight is 774.96. Indocyanine green is a dark, green-blue, odorless, lump, soluble in water or methanol and practically insoluble in acetone.
Indocyanine green is unstable in aqueous solution but is stable in the solutions containing protein or in the dry form. Its melting point is 230°C (decomposition). It has a pH of 5-7 and an osmotic pressure ratio to physiological saline of about 0.01 when 1 vial of Diagnogreen is dissolved in 5 mL water for injection (0.5% aqueous solution).
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