Serum or plasma Transfer 3 drops of specimen to specimen well & start timer.
Venipuncture whole blood Transfer 3 drops of specimen to specimen well then add 1 drop of buffer & start timer.
Fingerstick whole blood Fill tube & transfer approx 75 microliter of specimen to specimen well then add 1 drop of buffer to buffer well & start timer. Read results w/in 10-20 min.