Immunobladder Caution For Usage

Immunobladder is a freeze-dried preparation of BCG, an attenuated strain of Mycobacterium bovis. It is hardly considered that medical staff using Immunobladder will be infected with BCG under normal handling circumstances. However, Immunobladder should be handled using aseptic technique wearing rubber gloves and mask.
When damaged skin, such as injured fingers, accidentally made contact with Immunobladder, the surrounding region of skin should be immediately disinfected with rubbing alcohol.
All containers and equipment in contact with Immunobladder should be disinfected by boiling or soaking in antiseptic solution.
Precautions for handling: Reconstitute Immunobladder just before use.
Before adding the accompanying diluent, check the dryness of the product in the vial. Then remove the plastic cover from the top of vial and wipe the surface of gum-stopper and surrounding with a sterile cotton-piece soaked with alcohol. Wipe the neck of attached ampoule containing the accompanying diluent (isotonic sodium chloride solution, JP) with cotton-piece soaked with alcohol, and put the cut mark side up and snap it towards the opposite side to open.
Inject the diluent into the vial of Immunobladder and allow the solution to stand for about 1 minute, and then shake the vial gently to make a homogeneous suspension. Avoid foaming since this will make the withdrawal of the suspension difficult. Withdraw the entire suspension into the syringe and further dilute in additional isotonic sodium chloride solution, JP to prepare homogeneous BCG suspension for intravesical instillation.